CK-MB FS

December 24, 2024

Product Number: 1 1641

Description

Creatine kinase (CK) is an enzyme consisting of isoenzymes mainly in muscle (CK-M) and brain (CK-B). CK is found in the human body in dimeric form as CK-MM, CK-MB, CK-BB as a macro-enzyme. The CK-MB measurement is a specialized test for detecting cardiac muscle damage and, therefore, is used for the diagnosis and monitoring of myocardial infarction.

Features

Ready to use reagent.

Proline CK-MB FS comes in a liquid format which provides convenience for users as no reconstitution is required which helps reduce the risk of misdiagnosis.

Open system reagent, compatible with a wide range of instruments.

Proline CK-MB FS reagent is suitable for various third party analyzers such as Abbott, Advia, Cobas, Hitachi, Olympus, Response, TokyoBoeki, and Beckman Coulter. Please contact our technical support at marketing@proline.co.id for further compatibility information on other types of analyzers.

Specification

The UV optimization assay was optimized based on DGKC and IFCC homogeneous methods for CK by inhibiting the CK-M isoenzyme by monoclonal antibodies.
No interference: ascorbic acid 30 mg/dL, bilirubin 25 mg/dL, hemoglobin 10mg/dL, lipemia to triglycerides 1400mg/dL
Measurement linearity reaches 2000 U/L with a lower limit of detection of 4.3 U/L

Calibrator

TruCal CK-MB

Control

Kit Lines

No. Catalog	R1	R2	Kit Size
1 1641 99 10 021	5 X 20 mL	1 X 25 mL	Multi Purpose Kit
1 1641 99 10 026	5 X 80 mL	1 X 100 mL	Multi Purpose Kit
1 1641 99 10 921	4 X 21 mL	4 X 60 mL	Proline® R-910
1 1641 99 10 930	4 X 20 mL	2 X 10 mL	Hitachi 704/717/902/911/912/917
1 1641 99 10 964	6 X 16 mL	6 X 6 mL	Other Automated Instrument

Reference Range

The measurement of creatine kinase (CK) is specific for detecting heart muscle damage and is used for the diagnosis and monitoring of myocardial infarction.

Adults
< 24 U/L	< 0.4 µkat/L

Each laboratory should check if the reference ranges are transferable to its own patient population and determine own reference ranges if necessary.

References

Stein W. Creatine kinase (total activity), creatine kinase isoenzymes and variants. In: Thomas L, ed. Clinical laboratory diagnostics. Frankfurt: TH-Books Verlagsgesellschaft;1998.p.71–80.
Moss DW, Henderson AR. Clinical enzymology. In: Burtis CA, Ashwood ER, editors. Tietz Textbook of Clinical Chemistry. 3rd ed. Philadelphia: W.B Saunders Company; 1999. p. 617–721.
Würzburg U, Hennrich N, Orth HD, Lang H. Quantitative determination of Recommendations of the German Society for Clinical Chemistry. Standardization of methods for estimation of enzyme activities in biological fluids: standar method for determination of creatinine kinase activity. J. Clin Chem Clin Biochem 1977;15: 255-60
Shumann G, Bonora R, Ceriotti F, Férard G et al. IFCC primary reference procedure for the measurement of catalytic activity concentrations of enzymes at 37 °C. Part 5: Reference procedure for the measurement of catalytic concentration of creatine kinase. Clin Chem Lab Med 2002;40:635-42.
Stein W. Strategie der klinisch-chemischen Diagnostik des frischen
Myokardinfarkts. Med Welt 1985:36:572-7.
Myocardial infarction redefined – a consensus document of the Joint European society of Cardiology/America College of Cardiology Committee for the redefinition of myocardial Infarction. Eur Heart J 2000;21:1502-13.
Guder WG, Zawta B et al. The Quality of Diagnostic Samples. 1st ed. Darmstadt: GIT Verlag; 2001; p. 24-5.
Young DS. Effects of Drugs on Clinical Laboratory Tests. 5th ed. Volume 1 and 2.
Washington, DC: The American Association for Clinical Chemistry Press 2000.
Bakker AJ, Mücke M. Gammopathy interference in clinical chemistry assays:
mechanisms, detection and prevention. ClinChemLab 2007;45(9):1240–1243.