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PROLINE ALAT (GPT) FS (IFCC mod.)

Number Catalog : 12701

Product Description

Diagnostic reagent for the quantitative determination of ALAT (GPT) in serum or heparin plasma in vitro using a photometric system.

ALAT (GPT): Types and Benefits

Alanine aminotransferase (ALAT/ALT), previously known as Glutamic Pyruvic Transaminase (GPT), is the most important enzyme in the aminotransferase or transaminase group, which catalyzes the conversion of α-keto acids to amino acids through the transfer of an amino group. As a liver-specific enzyme, ALAT will only increase significantly in hepatobiliary diseases. The AST/ALT ratio is crucial for differential diagnosis in liver diseases and is used to distinguish damage to the liver, heart, or skeletal muscles.

The relationship between ALAT and liver damage (liver cirrhosis)

In liver cirrhosis, liver tissue is damaged and replaced by scar tissue (fibrosis), leading to portal hypertension. This high pressure forces blood to flow through alternative vessels, such as esophageal varices, which can rupture and cause bleeding. Additionally, alanine aminotransferase (ALAT) enzymes are released into the blood, indicating liver cell damage.

ALAT levels in the body increase during:

  • Hepatitis Virus
  • Toxic Hepatitis
  • Mononucleosis Infection
  • Cirrhosis
  • Polymyositis
  • Acute Pancreatitis

ALAT evels in the body decrease during:

  • End-stage liver disease
  • Renal hemodialysis and/or renal insufficiency

Measurement Method

The optimized UV test according to the IFCC (International Federation of Clinical Chemistry and Laboratory Medicine)

Product Advantages

  • Liquid reagent, open-system, ready to use without the need for reconstitution
  • Reagent functionality and installation testing guarantee
  • Good performance in linearity and stability
  • Available in MPK (multi-purpose kit) and dedicated kit types

Specifications

Sample type Serum or Plasma
Measurement range 3 U/L - 600 U/L
Analysis wavelength 340 nm
Analysis mode Kinetic
Reagent aspiration volume for manual instrument R1: 1000 µL | R2: 250 µL
Sample aspiration volume for manual instrument 100 µL
Storage temperature 2 – 8 °C
pH R1: 7,15 | R2: 9,6 - 9,7
Odor characteristics No distinctive odor
Visual characteristics R1: Colorless, clear | R2: Slightly yellowish, clear
On-board stability With P-5-P: 6 days Without P-5-P: 4 weeks
Expiration date 18 months

 

Calibrator

Control

Kit Lines

Catalog Number R1 R2 Kit
1 2701 99 10 022  5 X 20 mL 1 X 25 mL Kit Serba Guna (MPK)
1 2701 99 10 025 3 X 80 mL 1 X 60 mL Kit Serba Guna (MPK)
1 2701 99 10 029 3 X 200 mL 1 X 150 mL Kit Serba Guna (MPK)
1 2701 99 10 181 4 X 36 mL 4 X 9 mL TMS50i
1 2701 99 10 191 4 X 36 mL 4 X 9 mL TMS24i
1 2701 99 10 591 4 X 60 mL 4 X 15 mL Mindray
1 2701 99 10 914 6 X 60 mL 6 X 15 mL Hitachi 917
1 2701 99 10 920 4 X 34 mL 4 X 10 mL Proline® R-910
1 2701 99 10 921 4 X 21 mL 4 X 6 mL Proline® R-910
1 2701 99 10 951 6 X 36 mL 6 X 9 mL Advia
1 2701 99 10 965 6 X 25 mL 6 X 6 mL Instrumen lainnya

Reference Range

Woman <34 U/L <0,57 μkat/L
Man <45 U/L <0,75 μkat/L
Child <25 U/L - <30 U/L <0,42 μkat/L - <0,50 μkat/L

 

Interference

Interfering Substances Interference ≤ 10% up to <mg/dL> Analytical Concentration <U/L>
Ascorbate 30 121
Conjugated Bilirubin 50 49,8
55 93,8
Unconjugated Bilirubin 45 46,1
45 85,7
Hemoglobin 500 50,9
850 107
Lipemic (triglycerides) 1000 35,5
1000 114

 

Product Completeness

Name

  • Alanine Aminotransferase (ALAT/GPT) Reagent

Packaging

  • Doos
  • Kit insert
  • Reagents bottle

Journal

  • Thomas L. Alanine aminotransferase (ALT), Aspartate aminotransferase (AST). In: Thomas L, editor. Clinical
    Laboratory Diagnostics. 1st ed. Frankfurt: TH-Books Verlagsgesellschaft; 1998. p. 55-65.
  • Moss DW, Henderson AR. Clinical enzymology. In: Burtis CA, Ashwood ER, editors. Tietz Textbook of
    Clinical Chemistry. 3rd ed. Philadelphia: W.B Saunders Company; 1999. p. 617-721.
  • Bergmeyer HU, Horder M, Rej R. Approved Recommendation (1985) on IFCC Methods for the Measurement
    of Catalytic Concentration of Enzymes. L Clin. Chem. Clin. Biochem 1986; 24: 481-495.
  • Bakker AJ, Mücke M. Gammopathy interference in clinical chemistry assays: mechanisms, detection and
    prevention. ClinChemLabMed 2007;45(9):1240-1243.
  • Guder WG, Zawta B et al. The Quality of Diagnostic Samples. 1st ed. Darmstadt: GIT Verlag; 2001; 14-5.
  • Young DS. Effects of Drugs on Clinical Laboratory Tests. 5th ed. Volume 1 and 2. Washington, DC: The
    American Association for Clinical Chemistry Press 2000.

Download

  • Brochure : INA 

Related Product

Category

  • Liver disease

Order information

Contact our team to find out more product information and ordering

  • Telp : +62 21 8984 2722
  • WhatsApp :  +62 815 1359 2626
  • Email : marketing@proline.co.id

Technical Support

Contact our Technical support team for further assistance with product specifications, services and other technical documents.

  • Telp : +62-21-8984-2722
  • WhatsApp :  +62-817-9324-884
  • Email : technical.support@prodis.co.id
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