Alkaline Phosphatase FS (IFCC mod. 37°C)

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Alkaline Phosphatase FS (IFCC mod. 37°C)

November 15, 2024

Alkaline Phosphatase FS (IFCC mod. 37°C)

Product Number: 1 0441

Description

Alkaline Phosphatase (AP), a hydrolytic enzyme acting optimally at alkaline pH, exists in blood in numerous distinct forms which originate mainly from bone and liver, but also from other tissues as kidney, placenta, testes, thymus, lung, and tumors. Physiological increases are found during bone growth in childhood and in pregnancy, while pathological increases are largely associated with hepatobiliary and bone diseases. In hepatobiliary diseases, they indicate obstruction of the bile ducts as in cholestasis caused by gall stones, tumors, or inflammation. Elevated activities are also observed in infectious hepatitis. In bone diseases, elevated AP activities originate from increased osteoblastic activity as in Paget’s disease, osteomalacia (rickets), bone metastases, and hyperparathyroidism.

Increased in

Intrahepatic cholestasis, (example: liver infiltrates by carcinoma, leukemia, tuberculosis, sarcoidosis, amyloidosis, fibrosis (cirrhosis))
Extrahepatic cholestasis (eg, bile duct stones, neoplasms, atresia)
Osteoblastic disease, (eg malignancy, secondary hyperparathyroidism, Paget's disease)
Tumor
Pregnancy

Decreased in

Hypophosphatasemia: a relatively rare inherited disorder that is asymptomatic or may be associated with arthritis of the weight-bearing joints
Hypothyroidism
Scurvy
Severe anemia

Features

Ready to use reagent.

Proline Alkaline Phosphatase FS (IFCC mod. 37°C) comes in a liquid format which provides convenience for users as no reconstitution is required which helps reduce the risk of misdiagnosis.

Open system reagent, compatible with a wide range of instruments.

Proline Alkaline Phosphatase FS (IFCC mod. 37°C) reagent is suitable for various third party analyzers such as Abbott, Advia, Cobas, Hitachi, Olympus, Response, TokyoBoeki, and Beckman Coulter. Please contact our technical support at marketing@proline.co.id for further compatibility information on other types of analyzers.

Specification

Kinetic photometric test according to IFCC (International Federation of Clinical Chemistry and Laboratory Medicine)
No interference: ascorbic acid 30mg/dL, bilirubin 70mg/dL, lipemia triglyceride 2200 mg/dL, and hemoglobin 100mg/dL
Measurement linearity reaches 1400 U/L, with a lower limit of detection of 0.6 U/L

Calibrator

TruCal U

Control

Kit Lines

No. Catalog	R1	R2	Kit Size
1 0441 99 10 022	5 x 20 mL	1 X 25 mL	Multi Purpose Kit
1 0441 99 10 027	2 x 100 mL	2 X 25 mL	Multi Purpose Kit
1 0441 99 10 180	4 x 20 mL	4 X 5 mL	TMS50i
1 0441 99 10 190	4 x 20 mL	4 X 5 mL	TMS24i
1 0441 99 10 591	4 X 60 mL	4 X 15 mL	Mindray
1 0441 99 10 914	6 X 60 mL	6 X 15 mL	Hitachi 917
1 0441 99 10 920	4 X 34 mL	4 X 10 mL	Proline® R-910
1 0441 99 10 921	4 X 21 mL	4 X 6 mL	Proline® R-910
1 0441 99 10 951	6 X 36 mL	6 X 9 mL	Advia
1 0441 99 10 965	6 X 25 mL	6 X 6 mL	Other Automated Instrument

Reference Range

DGKC
			25°C	30°C	37°C
Children
1-12 year		[U/L]	< 480	< 596	< 727
		[µkat/L]	< 8.00	< 9.93	< 12.1
13-17 year	women	[U/L]	< 296	< 367	< 448
		[µkat/L]	< 4.93	< 6.12	< 7.47
	men	[U/L]	< 617	< 767	< 935
		[µkat/L]	< 10.3	< 12.8	< 15.6
Adults		[U/L]	< 170	< 211	< 258
		[µkat/L]	< 2.83	< 3.52	< 4.30
IFCC

Adults
Women	35 – 104 U/L		0.58 – 1.74 µkat/L
Men	40 – 129 U/L		0.67 – 2.15 µkat/L
Children	Women [U/L]	Women [µkat/L]	Men [U/L]	Men [µkat/L]
1 – 30 days	48 – 406	0.8 – 6.77	75 – 316	1.25 – 5.27
1 – 12 month	124 – 341	2.07 – 5.68	82 – 383	1.37 – 6.38
1 – 3 year	108 – 317	1.8 – 5.28	104 – 345	1.73 – 5.75
4 – 6 year	96 – 297	1.6 – 4.95	93 – 309	1.55 – 5.15
7 – 9 year	69 – 325	1.15 – 5.42	86 – 315	1.43 – 5.25
10 – 12 year	51 – 332	0.85 – 5.53	42 – 362	0.7 – 6.03
13 – 15 year	50 – 162	0.83 – 2.7	74 – 390	1.23 – 6.50
16 – 18 year	47 – 119	0.78 – 1.98	52 – 171	0.87 – 2.85

Reference

Thomas L. Alanine aminotransferase (ALT), Aspartate aminotransferase (AST). st In: Thomas L, editor. Clinical Laboratory Diagnostics. 1 ed. Frankfurt: THBooks Verlagsgesellschaft; 1998. p. 55-65.
Moss DW, Henderson AR. Clinical enzymology. In: Burtis CA, Ashwood ER, rd editors. Tietz Textbook of Clinical Chemistry. 3 ed. Philadelphia: W.B Saunders. Company; 1999. p. 617-721.
IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37°C. Part 9: Reference procedure for the measurement of catalytic concentration of alkaline phosphatase; Clin Chem Lab Med 2011;49(9).
Young DS. Effects of Drugs on Clinical laboratory Tests. 5th ed. Volume 1 and 2. Washington, DC: The American Association for Clinical Chemistry Press 2000.