Alkaline Phosphatase FS (IFCC mod. 37°C)
Alkaline phosphatase (AP) is a cell membrane-bound enzyme expressed by all tissues. AP, with its cofactors zinc and magnesium, catalyzes the hydrolysis of organic phosphate esters in the extracellular space. AP is found in the blood in various different forms, mainly from bones and liver, but also from other tissues such as kidneys, placenta, testes, thymus, lungs and tumors.
Increased AP activity can appear physiologically, for example during the second trimester of pregnancy and in childhood during growth. Pathological conditions that cause increased AP activity are hepatobiliary diseases, skeletal system diseases, malignant tumors, and systemic diseases without primary liver and bone involvement. Decreased AP activity in serum is very rare and, if present, is found in hereditary hypophosphatemia, Wilson's disease, and corticoid-induced osteoporosis.
| Alkaline Phosphatase levels in the body increase during: | Alkaline Phosphatase levels in the body decrease during: |
| Intrahepatic cholestasis, (example: liver infiltrate by carcinoma, leukemia, tuberculosis, sarcoidosis, amyloidosis, fibrosis (cirrhosis)) | Hypophosphatasemia: a relatively rare inherited disorder that is asymptomatic or may be associated with arthritis of weight-bearing |
| Extrahepatic cholestasis, (examples: bile duct stones, neoplasms, atresia) | Hypothyroidism |
| Osteoblastic diseases, (e.g. malignancy, secondary hyperparathyroidism, Paget's disease) | Scurvy |
| Tumor | Severe anemia |
| Pregnancy |
Proline Alkaline Phosphatase FS (IFCC mod. 37°C) comes in a liquid format which provides convenience for users as no reconstitution is required which helps reduce the risk of misdiagnosis.
Proline Alkaline Phosphatase FS (IFCC mod. 37°C) reagent is suitable for various third party analyzers such as Abbott, Advia, Cobas, Hitachi, Olympus, Response, TokyoBoeki, and Beckman Coulter. Please contact our technical support at marketing@proline.co.id for further compatibility information on other types of analyzers.
- The optimized UV test according to the IFCC (International Federation of Clinical Chemistry and Laboratory Medicine.
- No interference: bilirubin 70mg/dL, lipemia triglyceride 2200 mg/dL, and hemoglobin 100mg/dL
- Measurement linearity reaches 1400 U/L, with a lower limit of detection of 2.64 U/L
| Control for Alkaline Reagent | Calibrator for Alkaline Reagent |
| TruLab N | TruCal U |
| TruLab P |
| Catalogue Number | R1 Reagent Volume | R2 Reagent Volume |
| 1 0441 99 10 920 | 4 x 34 mL | 4 x 10 mL |
| 1 0441 99 10 921 | 4 x 21 mL | 4 x 6 mL |
| 1 0441 99 10 190 | 4 x 20 mL | 4 x 5 mL |
| 1 0441 99 10 022 | 5 x 20 mL | 1 x 25 mL |
| 1 0441 99 10 027 | 2 x 100 mL | 2 x 25 mL |
| 1 0441 99 10 180 | 4 x 36 mL | 4 x 9 mL |
| 1 0441 99 10 965 | 6 x 25 mL | 6 x 6 mL |
| 1 0441 99 10 914 | 6 x 60 mL | 6 x 15 mL |
| 1 0441 99 10 591 | 4 x 60 mL | 4 x 15 mL |
| Wanita | Pria | |||
| Anak-anak | [U/L] | [µkat/L] | [U/L] | [µkat/L] |
| 0-1 year | 89-370 | 1.49-6.3 | 89-370 | 1.49-6.3 |
| 1-3 year | 91-334 | 1.52-5.6 | 91-334 | 1.52-5.6 |
| 4-6 year | 97-316 | 1.61-5.3 | 97-316 | 1.61-5.3 |
| 7-11 year | 120-340 | 2-5.7 | 110-316 | 1.83-5.3 |
| 13-17 tahun | 49-328 | 0.82-5.5 | 75-363 | 1.25-6.1 |
| Dewasa | 33-98 | 0.55-1.64 | 43-115 | 0.72-1.92 |
- Thomas L. Clinical Laboratory Diagnostics [Internet]. Prof. Lothar Thomas; 2024. Available from: https://www.clinical-laboratory-diagnostics.com/
- Lowe D, Sanvictores T, Zubair M, et al. Alkaline Phosphatase. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan-.Available from: https://www.ncbi.nlm.nih.gov/books/NBK459201/
- IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37°C. Part 9: Reference procedure for the measurement of catalytic concentration of alkaline phosphatase; Clin Chem Lab Med 2011;49(9).
- Bakker AJ, Mücke M. Gammopathy interference in clinical chemistry assays: mechanisms, detection and prevention. ClinChemLabMed 2007;45(9):1240-1243.
- Guder WG, da Fonseca-Wollheim F, Heil W, Schmitt Y, Töpfer G, Wisser H, Zawta B. Quality of Diagnostic Samples. 3rd edition; 2010. p. 32-3.
- Young DS. Effects of Drugs on Clinical Laboratory Tests. 5th ed. Volume 1 and 2. Washington, DC: The American Association for Clinical Chemistry Press 2000.
- Young DS. Effects on Clinical Laboratory Tests - Drugs Disease, Herbs & Natural Products [Internet]. AACC Press and John Wiley and Sons, Inc; 2020. Available from: https://clinfx.wiley.com/aaccweb/aacc/
- Sonntag O, Scholer A. Drug interference in clinical chemistry: recommendation of drugs and their concentrations to be used in drug interference studies. Ann Clin Biochem. 2001 Jul;38:376-85.
- Liver Disease
- Bone Metabolism
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