PROLINE Total Protein FS
In vitro diagnostic reagent for quantitative examination of total protein in serum or heparin plasma using the photometric test method.
Total protein is the total amount of protein in serum/plasma, consisting of albumin and globulin, usually measured using the Biuret reaction method with a spectrophotometer because of its high accuracy and low interference.
Abnormalities in total protein levels can indicate a variety of medical conditions. Hypoalbuminemia or low total protein may occur in chronic liver disease, malnutrition, nephrotic syndrome, or severe bleeding. Conversely, hyperproteinemia may be seen in conditions such as multiple myeloma or chronic infections, in which immunoglobulin production is increased. Therefore, total protein testing is often used as an initial screening tool in the diagnosis of a variety of systemic diseases.
Photometric test according to the biuret method.
- Open-system liquid reagent, can be used directly without the need for reconstitution.
- Excellent linearity and stability performance.
- Available in MPK (Multi-Purpose Kit) and dedicated kit.
- Compatible with >65 brands of manual and automatic clinical chemistry analyzers.
| Sample type | Human heparin serum or plasma |
| Measurement range | 0.06 g/dL - 14 g/dL |
| Analysis wavelength | 540 nm, Hg 546 nm |
| Analysis mode | End-Point |
| Reagent volume used (analyzer manual) | 1000 µL |
| Sample volume used (analyzer manual) | 20 µL |
| Storage temperature | 2 – 25 °C |
| Open vial stability | 18 months |
| Expiration date | 24 months |
| Reference Range: | ||
| g/dL | ||
| Adult | 6,6 - 8,3 | |
| Children | Woman | Man |
| 1 - 30 days | 4,2 - 6,2 | 4,1 - 6,3 |
| 31 - 182 days | 5,6 - 7,9 | 5,5 - 7,0 |
| 1 - 18 years | 5,7 - 8,0 | 5,7 - 8,0 |
- Total Protein Reagent
- Doos
- Kit insert
- Reagents bottle
- Kit Insert PROLINE Total Protein FS-Ed.02 (10220 01 –Mar 2025/02)
- Sari, N. P. Y. P., Amril, A., Muttaminnah. (2024). Total Protein Examination Using the Biuret Method. International Journal of Scientific Advances (IJSCIA), Volume 5| Issue 6: Nov-Dec 2024, Pages 1081-1085.
- Loh TP, Leong SM, Sethi SK. High concentration of IgM‑κ paraprotein causes over‑estimation of serum total protein by certain biuret method. Clin Chem Lab Med. 2013;51(9):e205–7. doi:10.1515/cclm-2013-0160
- Brochure : INA
- Liver disease
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