PROLINE Bilirubin Auto Direct FS
| Catalogue Number | R1 Reagent Volume | R2 Reagent Volume |
| 10821 99 10 920 | 4 x 38 mL | 4 x 11 mL |
| 10821 99 10 921 | 4 x 23 mL | 4 x 7 mL |
| 10821 99 10 191 | 4 x 36 mL | 4 x 9 mL |
| 10821 99 10 181 | 4 x 36 mL | 4 x 9 mL |
| 10821 99 10 022 | 5 x 20 mL | 1 x 25 mL |
| 10821 99 10 965 | 6 x 25 mL | 6 x 6 mL |
| 10821 99 10 914 | 6 x 60 mL | 6 x 15 mL |
| 10821 99 10 951 | 6 x 36 mL | 6 x 9 mL |
| 10821 99 10 591 | 4 x 60 mL | 4 x 15 mL |
| 10821 99 10 027 | 2 x 100 mL | 2 x 25 mL |
Diagnostic reagent for the quantitative examination of direct bilirubin in human serum or heparin plasma in vitro using a photometric system.
Bilirubin is a yellow pigment and a breakdown product of hemoglobin that occurs in the liver and is excreted through bile. The measurement of total bilirubin in serum includes both direct (conjugated) and indirect (unconjugated) bilirubin. High total bilirubin levels may indicate health issues such as liver disease or hemolytic anemia.
- Total Bilirubin: Represents the sum of unconjugated and conjugated bilirubin in the blood. Total bilirubin testing typically requires a blood sample that must not be hemolyzed, as hemolysis can affect test results.
- Direct Bilirubin: Specifically measures the level of conjugated bilirubin in the blood. Direct bilirubin testing can be affected by sample storage time and temperature; delays in testing and improper storage temperatures may lead to changes in direct bilirubin levels.
Abnormal total bilirubin levels in the blood may indicate various medical conditions related to liver function, bile ducts, and red blood cell breakdown. Some of the diseases associated with bilirubin levels include:
1. Liver Diseases
- Hepatitis (viral, alcoholic, or autoimmune): Liver inflammation that disrupts bilirubin processing.
- Cirrhosis: Chronic liver damage that impairs bilirubin metabolism.
- Liver cancer (hepatocellular carcinoma): May cause increased bilirubin due to obstruction or liver cell damage.
2. Hemolytic Disorders
- Hemolytic anemia: Rapid breakdown of red blood cells increases unconjugated bilirubin.
- Sickle cell disease: A red blood cell disorder that leads to hemolysis and elevated bilirubin levels.
3. Bile Duct Diseases
- Gallstones (cholelithiasis): Cause bile duct obstruction, increasing direct bilirubin.
- Cholangitis: Bile duct infection that can raise total bilirubin levels.
- Pancreatic cancer: May block bile flow, leading to increased bilirubin in the blood.
4. Genetic Disorders
- Gilbert’s syndrome: A bilirubin metabolism disorder causing a mild increase in unconjugated bilirubin.
- Crigler-Najjar syndrome: A rare condition that results in increased unconjugated bilirubin due to a liver enzyme deficiency.
- Dubin-Johnson & Rotor syndromes: Bilirubin excretion disorders that cause elevated direct bilirubin levels.
Photometric test using 2,4-dichloroaniline (DCA).
- Ready-to-use liquid reagent (open-system) without reconstitution
- Excellent linearity and stability performance
- Available in MPK (Multi-Purpose Kit) and dedicated kit
- Compatible with >65 brands of manual and automated clinical chemistry analyzers
| Sample type | Human Serum or human plasma heparin. |
| Measurement range | 0.1 mg/dL - 7 mg/dL |
| Analysis wavelength | 546/658 nm |
| Analysis mode | End-Point |
| Reagent volume used (analyzer manual) | R1: 1000 µL ; R2: 250 µL |
| Sample volume used (analyzer manual) | 50 µL |
| Storage temperature | 2 – 8 °C |
| Open vial stability | 15 months |
| Expiration date | 18 months |
| Reference Range: | ||
| mg/dL | μmol/L | |
| Adult and Children | ≤ 0.2 | ≤ 3.4 |
- Bilirubin Reagent
- Doos
- Kit insert
- Reagents bottle
- Kit Insert PROLINE Bilirubin Auto Total FS - Ed.00 (Update: 10811 01 – Sep 2024/01)
- Bilirubin blood test. (n.d.-b). https://medlineplus.gov/lab-tests/bilirubin-blood-test/#:~:text=A%20bilirubin%20blood%20test%20measures,to%20help%20you%20digest%20food.
- Kesuma, S., Rodiyah, S., & Maulida, N. (2023). EVALUASI PEMERIKSAAN BILIRUBIN TOTAL MENGGUNAKAN MODEL SPECIMEN INSIGNIFICANT DAN MILD HEMOLYSIS. GEMA KESEHATAN, 15(2), 139–150. https://doi.org/10.47539/gk.v15i2.428
- Fajrian, F. M. (2020). Transferase enzymes with total bilirubin in patients with obstructive jaundice patients. In Jurnal Ilmiah Kesehatan Sandi Husada, Jurnal Ilmiah Kesehatan Sandi Husada (Vols. 11–11, Issue 1, pp. 176–182) [Journal-article]. https://doi.org/10.35816/jiskh.v10i2.240
- Hidayat, Fitriyani, N., & Yuliana. (2015). KORELASI ANTARA KADAR BILIRUBIN SERUM TOTAL DAN SERUM GLUTAMIC OXALOACETIC TRANSAMINASE PADA INFEKSI MALARIA FALSIPARUM DI RSUD. DR. H. ABDOEL MOELOEK PROVINSI LAMPUNG TAHUN 2015. Journal Kimia.
- The leukocytes and total bilirubin levels in obstructive jaundice caused by pancreatic tumors. (2020). In Jurnal Ilmiah Kesehatan Sandi Husada (Vols. 11–11, Issue 1, pp. 183–189) [Journal-article]. https://akper-sandikarsa.e-journal.id/JIKSH
- Grant LM, Faust TW, Thoguluva Chandrasekar V, et al. Gilbert Syndrome. [Updated 2024 Oct 5]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK470200/
- Brochure : INA
- Liver Disease
- Bone Metabolism
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