PROLINE Cholesterol FS
PROLINE Cholesterol FS is a colorimetric test based on enzymatic hydrolysis and oxidation for the determination of total cholesterol levels in serum and plasma. Cholesterol measurement is part of the standard lipid profile and is used for screening purposes in assessing atherosclerosis risk.
Cholesterol is an essential lipid component for the body, playing a crucial role in forming cell membranes and acting as a precursor for the synthesis of steroid hormones such as estrogen, testosterone, and cortisol. Cholesterol is also required for the production of bile acids, which aid in fat digestion. The human body obtains cholesterol from two main sources: synthesis by the liver and dietary intake from foods such as meat, eggs, and dairy products. In blood plasma, cholesterol is transported by lipoproteins, which form complexes between lipids and proteins.
The body contains four main types of lipoproteins: high-density lipoprotein (HDL), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), and chylomicrons. LDL, often referred to as "bad cholesterol," functions to carry cholesterol from the liver to body tissues, but excess LDL can form plaque in the blood vessels. In contrast, HDL, known as "good cholesterol," helps transport excess cholesterol from tissues back to the liver for disposal. VLDL is responsible for carrying triglycerides from the liver to tissues, while chylomicrons transport dietary fats after digestion.
Coronary artery disease (CAD) occurs when the coronary blood vessels become narrowed by atherosclerotic plaques formed primarily from cholesterol, especially LDL. This narrowing disrupts blood flow to the heart muscle, increasing the risk of heart attacks or heart failure. Risk factors such as high LDL levels, hypertension, diabetes, smoking, and an unhealthy diet exacerbate the condition. Preventing CAD can be achieved by adopting a healthy lifestyle, including a diet low in saturated fats, regular exercise, and, if necessary, medical treatment to lower cholesterol levels.
| Cholesterol levels in the body increase during: | Cholesterol levels in the body decrease during: |
| Familial Hypercholesterolemia (Inherited) | Severe Liver Insufficiency |
| Biliary Obstruction | Malnutrition |
| Nephrotic Syndrome | Hyperthyroidism |
| Hypothyroidism | Chronic Anemia |
| Pregnancy | Waldenström Macroglobulinemia |
| Thyroiditis |
Using the "CHOD-PAP" method with an enzymatic photometric test.
- Open-system liquid reagent, immediately usable without reconstitution.
- Guaranteed reagent functionality and installation testing.
- Excellent linearity and stability performance.
- Available in MPK (multi-purpose kit) and dedicated kit bottle.
| Sample type | Serum atau Plasma |
| Measurement range | 0,86 mg/dL - 750 mg/dL |
| Analysis wavelength | 546 nm |
| Analysis mode | End-Point |
| Reagent aspiration volume for manual instrument | R1: 1000 µL |
| Sample aspiration volume for manual instrument | 10 µL |
| Storage temperature | 2 – 8 °C |
| pH | 6.70 |
| Odor characteristics | Slight phenol odor |
| Visual characteristics | Transparant, pink |
| On-board stability | 8 weeks |
| Expiration date | 18 months |
| Control for Cholesterol Reagent | Calibrator for Cholesterol Reagent |
| TruLab N | TruCal U |
| TruLab P | Cholesterol Standard FS |
| TruLab L Level 1 | |
| TruLab L Level 2 |
| Catalogue Number | R/Mono Reagent Volume | Standard Volume |
| 1 1300 99 10 923 | 4 x 43 mL | - |
| 1 1300 99 10 192 | 4 x 60 mL | - |
| 1 1300 99 10 182 | 4 x 60 mL | - |
| 1 1300 99 10 022 | 6 x 20 mL | - |
| 1 1300 99 10 025 | 4 x 80 mL | - |
| 1 1300 99 10 029 | 4 x 200 mL | - |
| 1 1300 99 10 027 | 4 x 62.5 mL | 1 x 3 mL |
| 1 1300 99 10 023 | 6 x 20 mL | 1 x 3 mL |
| 1 1300 99 10 026 | 4 x 80 mL | 1 x 3 mL |
| 1 1300 99 10 019 | 2 x 50 mL | - |
| 1 1300 99 10 039 | 2 x 50 mL | 1 x 3 mL |
| 1 1300 99 10 024 | 5 x 50 mL | - |
| 1 1300 99 10 034 | 5 x 50 mL | 1 x 3 mL |
| Reference Range: | ||
| Reference | < 200 mg/dL | < 5,18 mmol/L |
| High-Risk Threshold | 200 – 239 mg/dL | 5,18 – 6,19 mmol/L |
| High Risk | ≥ 240 mg/dL | ≥ 6,22 mmol/L |
| Interfering Substances | Interference ≤ 10% up to (mg/dL) | Analytical Concentration (mg/dL) |
| Ascorbate | 6 | 222 |
| Conjugated Bilirubin | 15 | 147 |
| 25 | 236 | |
| Unconjugated Bilirubin | 21 | 149 |
| 23 | 237 | |
| Hemolysis | 230 | 152 |
| 230 | 223 | |
| Lipemia (Triglycerides) | 2200 | 136 |
| 2200 | 234 |
- Cholesterol Reagent
- Doos
- Kit insert
- Reagents bottle
- Rifai N, Bachorik PS, Albers JJ. Lipids, lipoproteins and apolipoproteins. In: rd Burtis CA, Ashwood ER, editors. Tietz Textbook of Clinical Chemistry. 3 ed. Philadelphia: W.B Saunders Company; 1999. p. 809-61.
- Recommendation of the Second Joint Task Force of European and other Societies on Coronary Prevention. Prevention of coronary heart disease in clinical practice. Eur Heart J 1998; 19: 1434-503.1.
- Artiss JD, Zak B. Measurement of cholesterol concentration. In: Rifai N, Warnick GR, Dominiczak MH, eds. Handbook of lipoprotein testing. Washington: AACC Press, 1997: p. 99-114.
- Deeg R, Ziegenhorn J. Kinetic enzymatic method for automated determination of total cholesterol in serum. Clin Chem 1983; 29: 1798-802.
- Schaefer EJ, McNamara J. Overview of the diagnosis and treatment of lipid disorders. In: Rifai N, Warnick GR, Dominiczak MH, eds. Handbook of lipoprotein testing. Washington: AACC press, 1997: p. 25-48.
- Guder WG, Zawta B et al. The Quality of Diagnostic Samples. 1 ed. Darmstadt: GIT Verlag; 2001. p. 22-3.
- Young DS. Effects of Drugs on Clinical laboratory Tests. 5th ed. Volume 1 and 2. Washington, DC: The American Association for Clinical Chemistry Press 2000.
- Bakker AJ, Mücke M. Gammopathy interference in clinical chemistry assays: mechanisms, detection and prevention. ClinChemLabMed 2007;45(9):1240-1243.
- Atherosclerosis
- Lipid Metabolism
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