Immunoturbidimetry is a powerful and precise method for determining the concentration of specific proteins in a sample. This technique utilizes the specific binding of an antigen and its corresponding antibody, which results in the formation of an immune complex. The agglutination caused by this interaction leads to a change in the turbidity of the sample, which directly influences the intensity of transmitted light. This change in light intensity is then measured photometrically and is directly proportional to the concentration of the protein in the sample.
Additionally, two distinct types of immunoturbidimetric testing can be performed: Direct and particle-enhanced immunoturbidimetry. Direct immunoturbidimetry is characterized by the direct binding of antibodies to their corresponding antigens, leading to the formation of the immune complex. This method is widely used in clinical and research settings, due to its high level of accuracy and sensitivity. The principle of particle-enhanced immunoturbidimetry utilizes particles coated with specific antibodies to form complexes with antigens present in a sample. This approach is particularly useful for detecting low concentrations of antigens. The use of microscopic particles amplifies the signal of these immune complexes, resulting in a significantly increased sensitivity in this method.
Immunoturbidimetry is a powerful analytical technique that combines the principles of turbidimetry and nephelometry to quantitatively determine the concentration of proteins in a sample of interest. Both methods rely on the measurement of light scattering, but employ different techniques to achieve this end. Turbidimetry measures the absorbance of light caused by the presence of particles in the sample, such as protein molecules. Nephelometry, on the other hand, measures the scattered light at a fixed angle, which is inversely proportional to the protein concentration in the sample. In the past, nephelometric assays were considered more sensitive compared to turbidimetric tests, but with the advent of innovative turbidimetric methodologies, such as those utilizing advanced optical instrumentation and sophisticated data analysis techniques, it has become possible to achieve equal sensitivity. As such, immunoturbidimetry is a highly reliable and robust analytical technique that is widely used in various applications, including clinical diagnostics, biotechnology, and biochemistry research.
Turbidimetry is a highly desirable method for various analytical applications due to its numerous benefits. One of the key advantages of turbidimetric testing is that it does not require the use of a dedicated analyzer. These assays can be easily performed using common photometric analyzers, eliminating the need for additional costs associated with purchasing specialized instrumentation or consumables. As a result, turbidimetry is an economical and efficient alternative to nephelometric testing. Furthermore, turbidimetry enables fully automated processing, eliminating the need for time-consuming sample splitting. This feature greatly increases the sample throughput, ultimately improving the overall efficiency of the laboratory. Overall, turbidimetry is a versatile, cost-effective, and efficient methodology that provides accurate results, making it an ideal choice for various analytical applications.
- Liquid-stable, ready-to-use reagents, calibrators and controls
- Long shelf life, on-board and calibration stability
- Wide measuring range combined with high prozone security
- Minimized interferences, advanced lipid-clearing system
- Fully quantitative results
- Traceability based on international reference material or method
- Convenient kits for automated systems and multi-purpose use
- Flexible applicability on clinical chemistry analyzers
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