PROLINE Triglycerides FS
Triglycerides FS is an enzymatic colorimetric test designed to measure triglyceride levels in serum and plasma. Triglycerides are evaluated to assess lipid status, detect atherosclerotic risk, and monitor lipid-lowering therapy. Elevated triglyceride levels, especially when combined with increased low-density lipoprotein (LDL) levels, pose a significantly high risk for coronary heart disease (CHD).
Triglycerides are esters formed from glycerol and three fatty acids, making them the most abundant type of natural fat. In plasma, triglycerides bind with apolipoproteins to form very low-density lipoproteins (VLDL) and chylomicrons. Hypertriglyceridemia (HTG) is a common condition, with a prevalence of approximately 10% in the adult population.
Measuring triglyceride levels is crucial for diagnosing HTG, as it is directly associated with an increased risk of atherosclerotic cardiovascular disease (ASCVD). Additionally, elevated triglyceride levels can contribute to the development of type 2 diabetes mellitus. At extremely high levels, triglycerides can trigger acute pancreatitis.
High triglyceride levels contribute to the development of coronary heart disease (CHD) through the mechanism of atherosclerosis, which involves the formation of plaques in the walls of blood vessels. Elevated triglycerides are often associated with increased very low-density lipoprotein (VLDL) and chylomicron remnants, which are atherogenic in nature. This condition is frequently accompanied by other risk factors such as low HDL levels, insulin resistance, and obesity, further increasing the risk of CHD. Therefore, controlling triglyceride levels is crucial in preventing CHD and other cardiovascular complications.
| Triglyceride levels in the body increase during: | Triglyceride levels in the body decrease during: |
| Hereditary hypertriglyceridemia | Malnutrition |
| Nephrotic syndrome | Congenital lipoproteinemia |
| Diabetes mellitus | 40 mini |
| Pancreatitis | |
| Pregnancy | |
| Liver disease (Zieve syndrome) | |
| Excessive alcohol consumption |
Using enzymatic colorimetry with glycerol-3-phosphate oxidase (GPO).
- Liquid reagent, open-system, ready to use without the need for reconstitution.
- Reagent functionality and installation testing guarantee.
- Good performance in linearity and stability.
- Available in MPK (multi-purpose kit) and dedicated kit types.
| Sample type | Serum atau Plasma |
| Measurement range | 3,08 mg/dL - 1300 mg/dL |
| Analysis wavelength | 546 nm |
| Analysis mode | End-Point |
| Reagent aspiration volume for manual instrument | 1000 µL |
| Sample aspiration volume for manual instrument | 10 µL |
| Storage temperature | 2 – 8 °C |
| pH | 7.20 |
| Odor characteristics | Slight phenol odor |
| Visual characteristics | Transparant, yellow |
| On-board stability | 4 weeks |
| Expiration date | 18 months |
| Control for Triglyceride Reagent | Calibrator for Triglyceride Reagent |
| TruLab N | TruCal U |
| TruLab P | Triglycerides Standard FS |
| TruLab L Level 1 | |
| TruLab L Level 2 |
| Catalogue Number | Reagent Volume | Standard Volume |
| 15710 99 10 923 | 4 x 43 mL | - |
| 15710 99 10 192 | 4 x 60 mL | - |
| 15710 99 10 182 | 4 x 60 mL | - |
| 15710 99 10 022 | 6 x 20 mL | - |
| 15710 99 10 025 | 4 x 80 mL | - |
| 15710 99 10 029 | 4 x 200 mL | - |
| 15710 99 10 027 | 4 x 62,5 mL | 1 x 3 mL |
| 15710 99 10 034 | 5 x 50 mL | 1 x 3 mL |
| 15710 99 10 019 | 2 x 50 mL | - |
| 15710 99 10 039 | 2 x 50 mL | 1 x 3 mL |
| 15710 99 10 023 | 6 x 20 mL | 1 x 3 mL |
| 15710 99 10 026 | 4 x 80 mL | 1 x 3 mL |
| 15710 99 10 024 | 5 x 50 mL | - |
| Reference Range: | ||
| Normal | < 150 mg/dL | < 1,65 mmol/L |
| Borderline | 150 – 199 mg/dL | 1,69 – 2,25 mmol/L |
| High | 200 – 399 mg/dL | 2,26 – 4,51 mmol/L |
| Very High | ≥ 400 mg/dL | ≥ 4,52 mmol/L |
| Interfering Substances | Interference ≤ 10% up to (mg/dL) | Analytical Concentration (mg/dL) |
| Ascorbate | 9 | 225 |
| Conjugated Bilirubin | 20 | 168 |
| 30 | 485 | |
| Unconjugated Bilirubin | 10 | 163 |
| 48 | 450 | |
| Hemolysis | 290 | 243 |
| 300 | 534 |
- Triglycerides Reagent
- Doos
- Kit insert
- Reagents bottle
- Rifai N, Bachorik PS, Albers JJ. Lipids, lipoproteins and apolipoproteins. In: Burtis CA, Ashwood ER, editors. Tietz Textbook of Clinical Chemistry. 3rd ed. Philadelphia: W.B Saunders Company; 1999. p. 809-61.
- Cole TG, Klotzsch SG, McNamara J. Measurement of triglyceride concentration. In: Rifai N, Warnick GR, Dominiczak MH, eds. Handbook of lipoprotein testing. Washington: AACC Press, 1997. p. 115-26.
- Recommendation of the Second Joint Task Force of European and other Societies on Coronary Prevention. Prevention of coronary heart disease in clinical practice. Eur Heart J 1998;19: 1434-503.
- Guder WG, Zawta B et al. The Quality of Diagnostic Samples. 1st ed. Darmstadt: GIT Verlag; 2001; p. 46-7.
- Young DS. Effects of Drugs on Clinical Laboratory Tests. 5th. ed. Volume 1 and 2. Washington, DC: The American Assoiation for Clinical Chemistry Press 2000.
- Bakker AJ, Mücke M. Gammopathy interference in clinical chemistry assays: mechanisms, detection and prevention. Clin Chem Lab Med 2007; 45(9):1240- 1243.
- Atherosclerosis
- Lipid Metabolism
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